h 293 T cells were transfected with plasmid encoding Flag-TBK1, together
Bafilomycin A1 (BafA1; Sigma‐Aldrich) was used at a final concentration of
Improvement of ACK1-targeted therapy efficacy in lung adenocarcinoma using chloroquine or bafilomycin A1 Mol Med
In order to investigate this combination effect, HepG2 cells were treated with bafilomycin A
First, we examined, side by side, three compounds that are widely used to reveal basal autophagy by blocking maturation of autophagosomes: bafilomycin A 1 (BafA1), chloroquine and vinblastine
Notably, due to its ability to specifically target V-ATPase and hence disrupt autophagic flux, BafA1 is frequently used to study autophagy and endosomal acidification
Bafilomycin A1 has been used to study the autophagy as an inhibitor of autophagosomes-lysosomes fusion and as an inhibitor of lysosomal degradation
, 2008b)
As expected, Torin 1 treatment induced SQSTM1/p62 degradation ( Figure 2
Data from the present study demonstrate that GFP-LC3 is degraded in a step-wise fashion in the autolysosome, in which the LC3 portion of the fusion protein appears to be more rapidly degraded than GFP
Similarly, treatment of cells with bafilomycin A1 or chloroquine reduced the efficiency of diC14-amidine based methods for gene delivery
14, 15, 33 At high doses (0
On the basis of Bafilomycin-A1, an autophagy inhibitor, enhances TQ cytotoxicity but does not promote apoptosis
Emre Bak